FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bs-25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bs-25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8454542
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT102050.12070435039532597No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG96500.1141398315840172No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT87600.10361294556227885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGT31200.025.204725
ACATGCG31550.025.1437514
TCGTATG8500.024.35960645
ATGCGTG33650.023.5746026
CTCGTAT8400.023.41712244
CTGACAT38450.022.3469281
TGCGTGC36550.022.0815757
GTCGCTA46400.021.33934627
CGCTATG46250.021.33471929
TGCGAGT38100.021.0021911
GTGCGAG39400.020.39676510
TATGCCG9900.020.21729748
TATCTCG10200.019.62069541
CGGGACT21500.019.4205591
GCTATGA51150.019.2258630
AACGCTG12900.018.98581922
CGGAATA28800.018.80776468
GCCAGTA19950.018.507841
GTCACGT11050.018.42138929
TAAGTCG17000.018.0568799
Produced by FastQC (version 0.11.2)