FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bs-25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bs-25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10731362
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT123690.11526029967118806No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG119030.1109178872169255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA25550.028.4935821
ATGCGTG36100.026.2732016
ACATGCG37600.025.500254
GTCACGT12500.024.56537229
CATGCGT39450.024.3044225
AGTCACG13250.023.43505128
GTCGCTA52000.022.62403727
CTGACAT45100.022.3389021
CGCTATG53550.021.90598529
ACCTAGG22700.021.87883210
GCTATGA55950.021.09142130
TGCGTGC46300.020.6341387
CTCGTAT13250.020.57604244
TAGGTAC24050.020.50739713
TACCTAG24150.020.1367559
TGCGAGT48100.019.9336711
ACGTCTG17500.019.90595415
CAACGTC61300.019.3019923
GTGCGAG49050.019.19601810
ATACCTA26650.019.1536718
Produced by FastQC (version 0.11.2)