FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bs-22_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bs-22_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602468
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG27810.10686010356323306No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG26050.10009729226257537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2350.024.96578446
GCCAGTA8050.024.0038641
ATGCGTG10050.022.3071636
CTGACAT10400.022.229541
TCGTATG2650.022.1373443
TGCGAGT10300.021.76572811
CATGCGT10350.021.660585
ACATGCG10350.021.660584
CTCGTAT2650.020.83554542
TGCGTGC10950.020.1587187
CGTAACG5400.019.8106542
CGGGACT5400.019.8087441
ACCTAGG4950.019.50966810
TAAGTCG4450.019.3765759
GTGCGAG11500.019.19460510
GCGTAAC5850.018.87483441
TACCTAG5700.018.7578879
AGTCGAC13150.018.09792315
TTCGTAC10300.018.08438120
TAGGTAC5350.018.05100313
Produced by FastQC (version 0.11.2)