FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_4bs-22_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_4bs-22_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306531
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA7300.029.7747961
CTCGTAT2250.029.1410942
CGCGAAT5150.024.7870921
CATGCGT8300.022.8531575
ACATGCG8100.022.565894
CTACACG2005.2750693E-1122.4168712
TAGTCAT9750.021.9305086
TCGTATG3000.021.85581843
GCGAATC6450.021.388522
ATGCGTG8600.020.8529036
TATGCCG3150.020.81506346
TGCGAGT8750.020.49542211
GTCGCTA9550.020.23048827
CGCTATG9750.019.81679229
CTGACAT9450.019.714831
CGGGACT4550.019.714831
ACTTAGG3550.019.44052932
CGAATCC7750.019.135413
TAAGTCG3250.019.1007659
GGTAACT10750.018.6148769
Produced by FastQC (version 0.11.2)