FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_3bw-28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_3bw-28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8301944
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG146260.1761756041717458No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG144590.17416402712424944No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT129650.15616824204065938No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC105510.12709071513852657No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT103910.1251634556918235No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT103840.12507913809103025No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT103660.12486232140327615No Hit
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA103380.12452505100010311No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG102290.12321210550203664No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG101740.12254961006723245No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA93490.11261217854516967No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTG86910.10468632407060323No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG85530.10302406279782181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA24550.044.2716671
TATGCCG4150.044.0743846
CTCGTAT4650.038.5925642
TCGTATG4750.037.78054443
TTCGATC13100.037.123132
CTGACAT32150.036.918521
CGTGAAC12500.033.12164364
CATGCGT35000.033.1094745
ACATGCG35600.032.5514534
GGTGGAT22800.032.522563
ATGCGTG35800.032.2732626
GGCAACG10850.031.1525172
GATCCTG16350.031.0084925
CTATCTC5800.030.93555338
TGCGTGC38150.030.3756687
CCTTACC7950.029.9466881
GGCTCAG16400.029.6521611
GTCGAAT3100.028.9386631
GTGCGAG40250.028.53378910
CAGTCAC6650.028.5331927
Produced by FastQC (version 0.11.2)