FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_3bw-25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_3bw-25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8613671
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG164210.19063881125712834No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG132590.15392972403984317No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT121020.14049758807830018No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG115890.13454193920338958No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT113040.1312332453839948No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG111070.12894618334041316No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT107770.12511506418111396No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC100020.11611773888275975No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA92500.10738743098035669No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG90310.10484496099282177No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGCCGACCCTGATCT90060.10455472469287484No Hit
GGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGAC88520.10276686908520188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA23300.041.7610471
CTGACAT35850.033.9753381
ACATGCG37650.031.4210554
TACGTAA6300.030.67155336
ATGCGTG38600.030.290336
CATGCGT39400.030.0254485
CGTAATC6350.029.34172238
CGTGAAC12900.028.08423864
TATGCCG7300.027.41962648
TTCGATC12850.027.3782882
TGCGTGC42950.027.302817
CTCGTAT7400.025.65074544
GGTGGAT18300.025.4433483
TAGTCAT38400.025.2386846
GTGCGAG47250.024.89110410
ACATCCG38500.024.4666855
TGCGAGT48400.024.22842411
CGGGACT25150.023.734841
CTGGTCG41700.022.8335369
TCGTATG8750.022.48204645
Produced by FastQC (version 0.11.2)