FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_3bw-22_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_3bw-22_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15017739
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG228420.15210012639053055No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG216510.14416950514321764No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT183540.1222154679875579No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG174800.1163956837976742No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC166940.11116187330196643No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT165570.11024961880080617No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG164380.1094572225552728No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT160450.10684031730741891No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA150180.1000017379447066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA40850.039.365351
CTGACAT55500.031.2126711
ACATGCG55950.029.7120694
CATGCGT57750.028.8456975
ATGCGTG57700.028.7511486
GGTGGAT42700.027.1392173
CGTGAAC17200.026.27790664
TGCGTGC64650.025.8737357
TAGGTAC47400.024.4483613
TTCGATC26950.024.4444772
ACCTAGG47200.024.3325810
TACCTAG48350.024.0391629
CGGGACT41750.023.7216931
GTGGATA49000.023.5091174
GTGCGAG70700.023.3669510
GGATACC49800.023.3392286
TAGTCAT68800.023.1600486
ACATCCG57300.022.5807155
TGCGAGT75000.022.25717411
GTACCCA52100.022.11096216
Produced by FastQC (version 0.11.2)