FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_3bs-25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_3bs-25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6043857
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT98940.1637034099251521No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG98760.16340558686282616No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT88410.14628076077908528No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT87220.1443118194225972No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG74730.12364620804231471No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG65500.10837450323526847No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC65270.10799395154451868No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG60760.10053182926068567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5200.041.14809446
CTCGTAT5850.037.1647942
TCGTATG6350.033.15149743
CTGACAT26600.031.9114511
GTCACGT6750.031.6908829
AGTCACG6950.031.27482828
ACATGCG27400.030.0854
CATGCGT27550.029.5456145
GCCAGTA18400.028.8799211
ATGCGTG29250.027.7105186
TGCGTGC30450.026.6184777
ACGTCTG9200.026.24339715
GGTGGAT13900.025.5578943
ATGCCGT8300.025.36373347
ACGTAGA8500.025.16649632
CAGTCAC8950.025.05578227
GTATGCC8650.024.73622345
GTGCGAG33000.024.56159610
TGCGAGT34550.023.55952811
CGGGACT22750.023.0544781
Produced by FastQC (version 0.11.2)