FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_3bs-22_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_3bs-22_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777183
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG36610.13182422620331466No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG32430.1167730034354956No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT30750.11072370816039129No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC29160.10499848227502474No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT29000.104422358915491No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG27820.10017344913892962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA8400.041.0772061
CATGCGT9650.032.523615
CTGACAT9950.031.9039861
ACATGCG10150.030.5816694
ATGCGTG10500.029.8907496
TGCGTGC11600.027.6509237
GTCCCTA7950.026.0296788
GTGCGAG11800.026.01313210
TAGTCAT13250.025.7693826
TGCGAGT12350.025.1339211
CCTATTA8400.025.0458211
CTCGTAT1801.2732926E-1124.9251142
CTATTAA8650.024.7206712
GGTGGAT7900.023.1384093
TTCGATC6600.022.4703182
TCGTATG2005.2750693E-1122.43381143
CTATAAG9700.022.41602346
ACATCCG9700.022.05623855
TCATATG15650.021.8175269
AGTCGAC13950.021.7574915
Produced by FastQC (version 0.11.2)