FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir64_3bs-22_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir64_3bs-22_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293986
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA6600.027.7164651
CGGGACT5250.026.2970241
GTCGCTA9350.023.2458527
CATGCGT7500.022.9938455
ACATGCG7550.022.841574
CGCTATG9600.022.64147629
CTATAAG7950.022.57328446
ATGCGTG8050.022.279756
TATAAGC8250.022.17075547
CGCGAAT6550.021.6047121
ACCTAGG6250.021.5222410
GTGGATA6600.020.9034984
ACATCCG8500.020.7016855
CTCGTAT1851.1737939E-820.51965342
TACCTAG6650.020.2276699
GCTATGA11200.020.02482230
TAGGTAC6750.019.92800113
CTTAAAC5650.019.548231
CTGACAT9600.019.4145981
TATCTCG3200.019.4010565
Produced by FastQC (version 0.11.2)