FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bw-28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bw-28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4132101
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG109600.26524037045561083No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT94670.22910863021015215No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG76860.1860070700111154No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT67690.16381496967281295No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG66080.15991864671265296No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG60930.1474552533928866No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC58810.14232469148261379No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT58210.1408726456589517No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG57540.13925119448919568No Hit
GGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGAC52180.12627958513114756No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAGGCCCCGCTTCCGACTCACGGAATAA49020.11863214379319383No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGCCGACCCTGATCT47870.11584905596450812No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC46160.11171072536707113No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA44460.10759659553336183No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG44170.10689477338525848RNA PCR Primer, Index 48 (100% over 43bp)
GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCG43020.1041116855565728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6700.049.44727746
TCGTATG7100.047.14871643
GCCAGTA10750.046.8632621
CTCGTAT7050.046.0134442
ATGCCGT7350.045.0743947
GCCGTCT7800.043.35461849
GGCAATC7600.042.22432736
ACGTCTG8250.041.8086315
GTATGCC8250.040.5769645
TCACTCG8300.040.32324630
CTGACAT20850.039.7185441
GTCACTC8500.039.3706529
TGCCGTC8650.039.098648
CACGTCT8900.038.7528514
CAGTCAC8650.038.68651627
GCAATCT8400.038.20111537
CAATCTC8450.037.97553338
ACACGTC9450.036.1324213
AGTCACT9300.035.9834928
AAAAAGG9200.035.24877569
Produced by FastQC (version 0.11.2)