FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bw-28_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bw-28_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9176952
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG214790.23405374682138472No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT200530.2185148184277307No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT150320.16380166312300642No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG146380.1595082986159239No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG134020.14603977442619293No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG124970.1361781122969805No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT124740.13592748441966354No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG122280.13324685581879472No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC120770.13160142931988747No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC116330.12676322160124626No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAGGCCCCGCTTCCGACTCACGGAATAA108890.11865595461325286No Hit
GCGGTTATGAGTACGACCGGGCGTGGACGGTACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGA104740.11413375595731567No Hit
GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCG103730.11303317266996712No Hit
GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACA100020.10899043604020157No Hit
GTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTG95090.10361828197423284No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGCCGACCCTGATCT94390.10285550147805067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA22550.043.920361
CTGACAT46850.038.0076181
ACATGCG52800.033.313714
CATGCGT53000.033.122925
ATGCGTG55050.032.0147636
TGCGTGC58650.030.0496647
TTCGATC13000.029.9799772
GGTGGAT16650.028.3788913
ACATCCG42150.028.1599755
GTGCGAG63950.027.45136610
CTGGTCG43400.026.9484669
CCCTGGT44600.026.61161467
CGGGACT36550.026.24751
TGCGAGT67200.026.22638111
GATCCCT45550.025.9812864
TAGTCAT38800.025.5115556
TATAAGC46900.025.38566847
CTATAAG46550.025.35413446
TAAGCAA47700.024.8843149
GGCTCAG15700.024.82364511
Produced by FastQC (version 0.11.2)