FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bw-25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bw-25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5002130
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT83000.1659293141121882No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG80200.16033169869635536No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG73220.14637764312402918No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG65830.13160393672295603No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG56260.11247208689098442No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT52330.10461543382519047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA18350.036.2964971
ACATGCG19750.028.1149544
CATGCGT19700.028.011245
CTCGTAT4100.027.77596742
CTGACAT21150.027.4121071
ATGCGTG20300.027.0134266
GGTGGAT18250.025.7012733
TCGTATG5050.025.28472143
TGCGTGC21650.025.1696857
TATGCCG4450.024.81634346
TAGTCAT26700.024.6717916
ACATCCG18550.024.55272355
CGGGACT15200.024.2931391
GTGGATA19000.024.1421874
TTACGGG4600.023.9922378
TGCGAGT23950.022.89655511
GTGCGAG24300.022.70870210
ACCTAGG20450.022.59904510
TACCTAG20700.022.4927229
CGCTATG30300.022.31764229
Produced by FastQC (version 0.11.2)