FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bw-22_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bw-22_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442699
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG64530.11856249996555017No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG64220.11799292961084198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA13500.033.2248651
ACATGCG17450.030.4371034
ATGCGTG18850.028.1765256
CATGCGT18950.027.8458375
CTGACAT21650.025.3391971
TGCGTGC22400.024.0189847
TGCGAGT22250.023.56088611
GTGCGAG22650.023.29706810
CGGAATA15150.021.40646468
TTCGATC12500.020.9693812
AGTCGAC26600.020.87575715
TATCTCG7500.020.6932775
CGGGACT11900.020.5856691
GAGTCGA26200.020.27222614
TAGTCAT22700.020.2070926
GTCGCTA22450.019.66995427
CGCTATG22650.019.65038929
GGCTCAG13850.019.42329411
CCTTACC7750.019.1434571
CGTGAAC9450.018.98598564
Produced by FastQC (version 0.11.2)