FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bw-22_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bw-22_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6120470
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG82930.13549613019915135No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT64740.10577619039060726No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT63610.1039299269500545No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG62720.10247579025793772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA16750.031.3154261
ACATGCG23250.028.7786584
ATGCGTG23800.027.6788586
CATGCGT24400.027.1395845
CTGACAT27650.025.4603611
TGCGTGC27250.024.6808387
CGGGACT13200.022.482991
TTCGATC13200.022.4721532
GTGCGAG28950.022.39757510
TGCGAGT29700.021.9481111
ACATCCG19150.021.08093355
CTATAAG20050.020.48302546
AGTCGAC33350.019.85657115
TAGTCAT26400.019.727176
GAGTCGA33400.019.61999514
ACCTAGG16250.019.52658310
TATAAGC21250.019.32633647
TAGGTAC16000.018.96943913
TACCTAG16850.018.8312749
CTCGTAT6200.018.36892544
Produced by FastQC (version 0.11.2)