FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bs-28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bs-28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16718981
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG306610.1833903633241763No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT264150.15799407870611254No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG214210.12812383721232773No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG209670.1254083607128927No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG209250.12515714923056614No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTG199410.11927162307320047No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT198340.11863163191584464No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT192510.11514457729212084No Hit
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA186990.11184294066725717No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC186890.11178312840956037No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT177550.10619666354067869No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG176110.10533536702984471No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC171210.10240456640270121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA39950.040.5089231
TTCGATC33600.037.3658522
CTGACAT67800.035.1167261
GGTGGAT46000.034.1896863
CATGCGT72400.032.4888155
ACATGCG73300.032.1369634
ATGCGTG76200.031.0044256
GATCCTG43500.030.36675
TGCGTGC79100.029.7369217
GGCTCAG43800.027.95389611
GTGGATA58350.026.89424
GTGCGAG88100.026.73824510
TGCGAGT92050.025.85314611
CTGGCTC47400.025.8308129
CCTTACC16450.025.591081
CGCTATG90250.024.42818329
TAGTCAT65850.024.3024626
ACATCCG61200.024.29919855
AACGCTG50100.023.96042622
GTCGCTA92400.023.7833227
Produced by FastQC (version 0.11.2)