FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bs-28_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bs-28_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24408115
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG464360.1902482022884602No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT393680.16129061994340815No Hit
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA386050.15816461041747795No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG381270.15620624534094502No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG332630.13627844673789843No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG321860.1318659798185972No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTG302000.12372934165542895No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT297080.12171361860594315No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC294050.1204722281913208No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT289990.11880884697568822No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC273440.11202831517304798No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG270580.11085657372558266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA68200.043.157421
TTCGATC45550.037.17942
CTGACAT94100.036.742521
GGTGGAT81950.035.0163083
CATGCGT104950.032.0416955
ACATGCG105700.031.781714
CCTTACC28000.031.4248351
CTCGTAT11450.031.04324542
ATGCGTG109900.030.6926566
TATGCCG12500.029.54063446
TGCGTGC114400.029.515497
GATCCTG60750.029.1816715
GGCTCAG59550.028.6113611
TCGTATG13000.028.1385943
TAGTCAT104900.027.6183136
CTGGCTC63400.026.8195179
GTGGATA107900.026.626674
GTGCGAG129900.025.91397310
ACATCCG94650.025.51812255
CGGGACT85600.025.2343081
Produced by FastQC (version 0.11.2)