FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bs-25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bs-25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8144474
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG94090.11552618376582699No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG93870.11525606196299477No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT90710.11137613061322316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA23200.033.9109951
CTCGTAT6850.027.70804242
ACATGCG25800.026.0670934
CATGCGT26000.025.9992265
ATGCGTG26650.025.4945136
CTGACAT28300.025.117391
TAGTCAT32750.024.115816
TGCGTGC28500.023.9606197
TCGTATG7550.023.76815443
TTCGATC19900.023.5713712
TATGCCG7850.023.30014646
TGCGAGT30150.022.53494811
GTCCCTA25650.021.782388
TAAGTCG14750.021.5116379
GTGCGAG31400.021.41818410
CCTATTA26700.021.05494111
CCTTACC11800.021.0544621
GGTGGAT25500.020.6933883
CTATTAA28050.020.41046512
CGCTATG34200.019.97515329
Produced by FastQC (version 0.11.2)