FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bs-25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bs-25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11945375
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT157650.13197576467879826No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG153690.1286606741102728No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG149620.12525349769262162No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC137240.1148896539455647No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT133260.11155782049538002No Hit
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA125800.1053127256364911No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT122990.10296035076337076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8100.037.06635344
GCCAGTA35700.034.2183341
TCGTATG8950.033.9315345
TATGCCG9450.031.40571248
CTGACAT45050.029.5676141
CATGCGT44900.028.7285
TTCGATC24650.028.2638262
ACATGCG45700.028.2251034
ATGCGTG46000.028.0410276
GGTGGAT35900.027.2838633
TGCGTGC48450.026.5518747
GATCCTG30000.025.061985
GTGCGAG51300.024.87508210
GTGGATA40000.024.4871644
TGCGAGT52950.024.36047711
GGCTCAG28200.024.21565411
ATGCCGT12750.023.81630749
GGCAACG19600.023.7560462
TAGTCAT53000.023.4915376
CGTGAAC19300.023.24044264