Basic Statistics
Measure | Value |
---|---|
Filename | HLNNLBGX5_n01_ir108_4bs-25_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11945375 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT | 15765 | 0.13197576467879826 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 15369 | 0.1286606741102728 | No Hit |
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG | 14962 | 0.12525349769262162 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC | 13724 | 0.1148896539455647 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 13326 | 0.11155782049538002 | No Hit |
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA | 12580 | 0.1053127256364911 | No Hit |
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT | 12299 | 0.10296035076337076 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 810 | 0.0 | 37.066353 | 44 |
GCCAGTA | 3570 | 0.0 | 34.218334 | 1 |
TCGTATG | 895 | 0.0 | 33.93153 | 45 |
TATGCCG | 945 | 0.0 | 31.405712 | 48 |
CTGACAT | 4505 | 0.0 | 29.567614 | 1 |
CATGCGT | 4490 | 0.0 | 28.728 | 5 |
TTCGATC | 2465 | 0.0 | 28.263826 | 2 |
ACATGCG | 4570 | 0.0 | 28.225103 | 4 |
ATGCGTG | 4600 | 0.0 | 28.041027 | 6 |
GGTGGAT | 3590 | 0.0 | 27.283863 | 3 |
TGCGTGC | 4845 | 0.0 | 26.551874 | 7 |
GATCCTG | 3000 | 0.0 | 25.06198 | 5 |
GTGCGAG | 5130 | 0.0 | 24.875082 | 10 |
GTGGATA | 4000 | 0.0 | 24.487164 | 4 |
TGCGAGT | 5295 | 0.0 | 24.360477 | 11 |
GGCTCAG | 2820 | 0.0 | 24.215654 | 11 |
ATGCCGT | 1275 | 0.0 | 23.816307 | 49 |
GGCAACG | 1960 | 0.0 | 23.756046 | 2 |
TAGTCAT | 5300 | 0.0 | 23.491537 | 6 |
CGTGAAC | 1930 | 0.0 | 23.240442 | 64 |