FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bs-22_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bs-22_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6162806
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG74690.12119479341066391No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG66970.1086680320620185No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT65670.10655860333750568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA16700.031.8212531
CATGCGT21400.026.9162675
CTGACAT23450.026.634761
ACATGCG22200.026.101684
CTCGTAT4050.025.56108542
ATGCGTG23700.024.7407446
TATGCCG4350.024.59112746
CCTTACC8250.023.4232481
TGCGTGC25150.022.9029067
TCGTATG5000.022.77492743
CGGGACT14550.022.0563161
TGCGAGT26050.021.97923311
TTCGATC15400.021.9498522
GGCTCAG15350.021.57123611
GATCCTG18350.021.245
GTGCGAG27150.020.83464810
GTCCCTA19250.020.7844928
CGGAATA17450.019.77119868
GTTAGTT16250.019.7488861
AACGCTG16000.019.40430522
Produced by FastQC (version 0.11.2)