FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_4bs-22_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_4bs-22_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4257161
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG46160.10842906810430708No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT44110.10361365238477005No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG43340.10180493526084637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA10150.031.6141971
TCGTATG2850.025.42853543
CTCGTAT2950.024.56626142
CATGCGT11950.023.9547565
ACATGCG12500.022.6248344
CGGGACT9950.022.1933121
GTTAGTT11150.021.9709431
ATGCGTG13150.021.7687726
TTCGATC10500.021.6803762
CTGACAT13750.021.3295381
TATGCCG3850.020.61742446
CGGAATA12400.020.31049568
GGCTCAG11150.020.1057411
TGCGTGC14350.019.9483877
CGCTATG15300.019.61765729
TAGTCAT16950.019.3301626
TGCGAGT15100.018.95757311
GATCCTG13600.018.7661155
GGTGGAT12600.018.613373
TTCGTAC18350.018.61002720
Produced by FastQC (version 0.11.2)