FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bw-28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bw-28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCAGTGACGAAGCCTAGGGCGTGAGCCCGGGTTGAACGGCCTCGAGTGCAGATCTTGGTGGTAGTAGCAAAT12.3255813953488373No Hit
CTTAACCTACCCAAATCCCACGACAACTAAATACTCACGCGCAAGAAACCATAAACGACATTACCACTGACCCAC12.3255813953488373No Hit
GGCGCGGGACTTCTGAATTTACAAATCGAAGGTCACCATACTAGACGAGATCCTCATCACATTTGAGAAGAGATT12.3255813953488373No Hit
AAACACACCTCTCCGCAAACTCGTAAGAAAATGTAAGCCGACTGACGCCTGCCCAGTGCCCCAAGGACAAGGAAC12.3255813953488373No Hit
ATTCACGGTTAGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGT12.3255813953488373No Hit
AGAATGTCGGCTTGAGTAACGAAAACATTGGTGACAATCCAAAGCCAAGAAAAACCAACGTTTCCTCCTCAAGGT12.3255813953488373No Hit
CTAACGTCGCCGTCAACCGCCACGTCCCGGCGCGGGAAATCTTCACCCGATTACCGTGCGGGGCACGCGCGTGGT12.3255813953488373No Hit
CCATATACCATAGACCTCATGCTTCAAGTTCTTAATAATGACCAATTCATCTTTCACCTCTACCCCCATGCACTC12.3255813953488373No Hit
GAATAAGCGACCCATCGCGTCGCTTGGGCCATACTCCGTTGCAAGCCGGATAGCGGGCGACCAGGAAGCGGTAGA12.3255813953488373No Hit
CACCTAAACCGCCCGCCAACACTCGAAACGCTACAACCTCAAGAAGTGACCCGCCACCCGAAGAGCACCGCACCT12.3255813953488373No Hit
CCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCGCTTGGTTGAAAAGC12.3255813953488373No Hit
GGGCTTCCTAACGTCGCCGTCAACCGCCACGTTCCGGCTCGGGAAATCTTAACCCGATTCCCTTTCGGGGCACGC12.3255813953488373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.3255813953488373No Hit
CAATCGTCTGCGTTACAAGGCAGGGAACGACCATGTAGAAGCTTGGATTTCTCTATCGGGCCATAACGCAAATGA12.3255813953488373No Hit
GGGGGGGGGGTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGCAGC12.3255813953488373No Hit
AAGCGGGACGTAGTCACTTCGTAAATCTTCCATTATTTTGGACATACGGCTCTGTTCTTGTTTTAAACTAGTGTA12.3255813953488373No Hit
GCGAAATGGGAGCAGCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAG12.3255813953488373No Hit
TGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGA12.3255813953488373No Hit
ATCGGAGATGTCGTAGGCGAGACCCTTCCACCTGTCCTGGTCGGTCTGCTTGCCCTCGTCCAGCTCCTTGGCCAT12.3255813953488373No Hit
CAAGGCACGTCGCTCAACGGATAAAAGTTACTCTAGGGAAAACAGGCTGATCTTCCACAAGATTCCACATCGACG12.3255813953488373No Hit
CCACGTCCCGGCTCGGGAAATCTTAACCCGATTCCCTTTCGGGGCACGCGCGTGGTCGCGCTCTCTGCCGGGGTT12.3255813953488373No Hit
GGAATATTATCCTTGGTATTCACCCTTTGATTACAACTTCCTAGGAACCGATGTCTACCAGACCTGGCAGCAAAT12.3255813953488373No Hit
GAGAACCATAAATGGTTCGGTAAACCACTACATTGCAGTTGAGTACGGCAGTCATCTAATAAGGTACGGTTTAGC12.3255813953488373No Hit
ACCCTAACTGTCGGCCGCAGAAACTACTACAGGCTCCCCGTGACACATCAGATTCCCCACGGACACCTTGCGTCC12.3255813953488373No Hit
ATTTGGTTCACTGCTTTAGGTATTAGTACTATGGCTTTCAATCTAAACGGATTCAATTTCAACCAATCTGTAGTT12.3255813953488373No Hit
GGCGATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCACTCGCGGTAATTGTTCGATATAG12.3255813953488373No Hit
GTCATGATTAAGGCATCCGAAGGTGGTGGTGGTAAAGGTATCAGACCTGTCTCTTATACACATCTCCGAGCCCAC12.3255813953488373No Hit
CTCCGGCTGAACCGTTTCCAGGGTTGGCGGGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGGCG12.3255813953488373No Hit
CCGGACTTCCTAACGTCGCCGTCAACCGCCACGTCCCGGCTCGGGAAATCTTAACCCGATTCCCTTTCGGGGCCC12.3255813953488373No Hit
AGGGACTCGGGCCTTAGAGCCAAAAGGAGGTGCCAGGCCCCGCTTCCGACTCACGGAATAAGTAAAATAACGTTA12.3255813953488373No Hit
CGCGAAGGGATACTGATTCAATAGTCATTACGTCGCCCCCCAGTCCATTAACCATCTACGGGGGTTGGCACGTGG12.3255813953488373No Hit
CCGGGGGCTCCCACTTATCCTACACCTCTCAAGTCATTTCGCAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCT12.3255813953488373No Hit
AATATCTTTGACTAAAAGCTGATAATCACAACAAGATGCATGTTATAAACAAAGTTATACCGTGCAGACCTTCAA12.3255813953488373No Hit
CCCGACTTCCCTAGCCTACATTGTTCCAATCGCCAGAGGGTCTTCACCTTGGAGACATCAGGGGGTTATGAGAAC12.3255813953488373No Hit
GTGCCGTTCACATGGAACCTTTCTCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGA12.3255813953488373No Hit
GGAAGACATTGTCAGGTGGGGATAAGTGGGAGCCCTCGGGCGCAAGTGAAATACCACTACTTTTAACGTTATTTT12.3255813953488373No Hit
GTGGGAACCGACTGATGAACTTGAATCTCGGGTACTGTACACGATACCGCTCGTAACCCTCTCGTACCCACAGGT12.3255813953488373No Hit
TGGCATTCAGAGCACTGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATT12.3255813953488373No Hit
TTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGT12.3255813953488373No Hit
CCACTTCCAAAAGGCAAACTCACAGATTTGACCAGGCCCAAACCAACGTAGACGCCAACTTCTAGATCCAGCCAA12.3255813953488373No Hit
GATTTCCGGAGGCGGGACGTGGCGGTTGACGGCGACGTTAGGAAGTCCGGAGACGCCGGCGGGGGCCTCGGGAAG12.3255813953488373No Hit
CTTCGTTGAAGAAGCAAATAAACAAAAGAAATCTTACATCGCCGCAGAGCTGAAAGCAACCATGTCATCGGCAGT12.3255813953488373No Hit
GTATTTGCCTCATCGGCATTCAAGGCAGACTTTAATGTCGATGCGTCCAAACTTTCAACTTTTACGTCTTCCTCA12.3255813953488373No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)