FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bw-25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bw-25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4476317
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG64610.14433740952662646No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG64160.14333211879319538No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT58990.13178244525577612No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT54200.12108168389325422No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC53970.12056786862950054No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT46990.10497469236428073No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG45730.1021598783106737No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA45120.10079715087202268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA14250.037.0462071
TATGCCG3100.032.28462646
TTCGATC7750.031.1508182
CTCGTAT3150.030.67486442
TCGTATG3200.030.19658343
GGTGGAT11900.029.2710193
CTGACAT17250.029.2032361
CATGCGT17400.028.3432855
ACATGCG17650.028.137224
ATGCGTG18350.027.4397536
GGCTCAG9600.026.94346611
TGCGTGC19300.025.7317077
ACCTAGG14200.025.25854910
ATGCCGT3700.025.18381147
GATCCTG11200.024.941955
GTGGATA14000.024.8803654
TACCTAG14450.024.821559
GGATACC15000.024.3712646
GCCGGTT1701.6734703E-1024.355641
TCAGGCG2850.024.2023892
Produced by FastQC (version 0.11.2)