FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bw-25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bw-25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894261
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG115770.16792227622365907No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG103150.1496171960997705No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT90830.13174726051131513No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT84490.12255120599582756No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT80560.11685081258165306No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG76430.11086032281052312No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA75080.10890217240107389No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC74670.10830747486931522No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG73960.1072776328021234No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT71450.10363692352233254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA18850.042.285221
CTGACAT28200.033.037161
ACATGCG28250.031.4990234
CATGCGT28650.031.0592445
ATGCGTG28650.030.938866
TGCGTGC31750.028.3525817
TAGTCAT30750.025.909726
GTGCGAG34900.025.29940810
TTCGATC11150.025.0563952
TGCGAGT35850.024.72519711
CTCGTAT5050.024.6004642
CGGGACT17900.024.2887691
CATATGC34200.023.69941110
GGTGGAT18450.022.8065343
GAGTCGA39000.022.5512914
AGTCGAC39550.022.50011815
CCTTACC8350.022.3149171
CGGAATA22200.022.06866668
ACATCCG24850.021.93718355
TCATATG36350.021.9181259
Produced by FastQC (version 0.11.2)