FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bw-22_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bw-22_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5408720
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG82350.1522541377627239No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG64440.11914094277389105No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT63230.11690381458089899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA14050.035.612011
CGTATCT3100.034.50584839
ACATGCG19400.029.8675424
CATGCGT20600.028.2951135
ATGCGTG20600.028.2951136
CTGACAT22000.027.6054461
TCGTATG4450.026.36670145
CTCGTAT4250.025.98327844
TTCGATC11650.025.4622362
TGCGTGC24500.023.7909937
TGCGAGT24950.023.22366111
GTGCGAG25950.022.46163210
CCGTATC4850.022.0544738
GTTAGTT14900.021.7693941
CCTTACC6000.021.2791981
TCCGTAT5050.021.18102537
GTCCCTA17950.020.9437278
CGGGACT12750.020.8394051
ACATCCG21450.020.42833755
TATGCCG5250.020.37714648
Produced by FastQC (version 0.11.2)