FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bw-22_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bw-22_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7478223
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG111980.14974145595818686No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG98460.13166229463871296No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT88990.11899885841863769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA21250.032.9613381
TTCGATC14550.032.9512142
CTGACAT30150.030.5556621
CATGCGT29000.030.2081015
ATGCGTG29300.029.7810886
ACATGCG29350.029.6128444
TGCGTGC31800.027.5482677
GTGCGAG34400.025.76691210
TGCGAGT34350.025.40279411
CCTTACC8300.023.2797181
GGCTCAG19550.022.22856711
AACGCTG19100.021.85174622
GTTAGTT23200.021.713641
AGTCGAC40600.021.57764615
GAGTCGA41350.021.26925814
GTGGATA23500.020.84054
GGTGGAT23200.020.8126683
GATCCTG23650.020.270825
TTAGGGT15500.020.248717
TAGTCAT33850.020.0722126
Produced by FastQC (version 0.11.2)