FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bs-28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bs-28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29476009
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT530580.18000401614750489No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG479710.16274591312548453No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG419610.14235644995223062No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC391310.13275542153620595No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT369000.12518655425841402No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT341050.11570426647650976No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG334990.11364835721145289No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG332170.11269164695939672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA85100.036.6942371
CTGACAT122300.031.8244921
CATGCGT123050.030.3550625
ACATGCG124000.030.233764
ATGCGTG130150.028.725626
TGCGTGC136950.027.3244887
CTCGTAT17900.027.18363642
TTCGATC56800.026.292772
GTGCGAG145300.025.70674710
TATGCCG20500.025.42026346
CGCTATG179350.025.1233729
TGCGAGT150100.025.09147811
TCGTATG20800.024.72145343
GGTGGAT79150.024.7067953
GTCGCTA184950.024.49142527
TAGTCAT127800.024.2882356
GTGGATA82900.023.381164
GCTATGA191350.023.35166730
CAACGTC199100.022.76544823
GGCTCAG63300.022.6659511
Produced by FastQC (version 0.11.2)