FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bs-28_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bs-28_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16623015
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG295590.17781972764868467No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT260280.15657809368517084No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG211280.12710088994084406No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT208090.12518186381952973No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT188390.11333082476313713No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG168140.10114891913410413No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG168050.10109477733130842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA47250.034.6900751
CTGACAT69850.031.617461
ACATGCG73300.029.5029454
CATGCGT74200.029.0521225
ATGCGTG77850.027.734326
TGCGTGC78950.027.1731517
TTCGATC37200.026.6110272
GTGCGAG86900.024.76661310
GGCTCAG38600.024.48302311
TGCGAGT89400.024.38267311
CGGGACT51600.023.3393731
GGTGGAT43650.022.9147723
AACGCTG40550.022.62820222
CGCTATG98000.022.53137229
TAGTCAT73400.022.1323056
GTCGCTA100850.022.03048727
GATCCTG48000.021.7003885
GTGGATA46500.021.4361444
GCTATGA104000.021.13336230
GTCGAAT10850.020.6726931
Produced by FastQC (version 0.11.2)