FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bs-25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bs-25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10489661
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG154110.14691609194996863No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG139850.13332175367726373No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC131250.12512320464884424No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT122050.11635266382774428No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT116600.11115707171089703No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA113810.10849730987493304No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT107730.10270112637577133No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG106640.10166200795240189No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG105830.1008898190322833No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT104980.10007949732598605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA33500.037.3905451
CTGACAT37750.029.981741
CTCGTAT6300.029.57792344
CATGCGT37500.029.0642495
ACATGCG38250.028.5845344
ATGCGTG38600.028.2359926
TCGTATG7200.027.79803745
TGCGTGC41650.026.0026687
GGTGGAT31350.025.9643673
TATGCCG7500.025.3060448
TTCGATC23400.025.0586152
TAGTCAT50700.024.6945976
TGCGAGT44600.024.59209611
GTGCGAG43700.024.54608510
GTGGATA34350.023.3955084
ACCTAGG34850.022.86190610
TACCTAG35950.022.8339659
GGCTCAG24100.022.7553411
GGATACC36900.022.24616
TAGGTAC36750.021.77378513
Produced by FastQC (version 0.11.2)