FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bs-22_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bs-22_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669177
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG48340.13174616542074694No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG45270.1233791664997355No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC41290.1125320473773819No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG37120.10116710096024259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA12400.030.3310891
CATGCGT12450.026.8713975
ATGCGTG12650.026.4465526
ACATGCG13050.025.3716414
CTGACAT14600.024.8153081
CCTTACC4850.023.477691
TGCGTGC14450.023.3908527
ACCTAGG11100.023.30376810
TACCTAG11250.022.9930529
TGCGAGT14950.022.60855311
TCGTATG2450.022.53608943
TAGTCAT16900.022.4488376
GTGCGAG14950.022.37785310
TTCGATC7900.022.266942
GTGGATA11300.021.9756584
GGTGGAT11400.021.782893
TAGGTAC11950.021.64617713
ATACCTA12200.021.4853088
CGCTATG16450.020.9737429
CGGGACT9450.020.4474621
Produced by FastQC (version 0.11.2)