FastQCFastQC Report
Fri 6 Apr 2018
HLNNLBGX5_n01_ir108_3bs-22_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNNLBGX5_n01_ir108_3bs-22_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705962
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG40350.10887861235490272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA11600.027.9622441
CATGCGT10300.025.4481265
ATGCGTG10750.025.0245136
ACATGCG10550.024.845094
ACCTAGG10300.023.77390910
ATACCTA11200.022.4793788
TCGTATG3400.022.33021443
TACCTAG11050.022.1602959
GGATACC11250.022.07296
CGCTATG14600.021.74007229
GTGGATA11000.021.6339514
TGCGTGC12400.021.41657
GTCGCTA14600.021.26631427
CTGACAT14250.021.067191
TAGGTAC11350.020.96682713
GTTAGTT8000.020.703961
CGGGACT8500.020.703961
TAGTCAT15250.020.5802696
CTCGTAT3700.020.519142
AGTCACG3650.019.84909428
Produced by FastQC (version 0.11.2)