FastQCFastQC Report
Sat 27 Feb 2021
HLN27AFX2_n02_AH10423_4h_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLN27AFX2_n02_AH10423_4h_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311278
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325630.9833967428889994No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC42030.1269298440058491No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT41420.12508765497792695No Hit
AGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAA39400.11898729131169296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)