FastQCFastQC Report
Sat 27 Feb 2021
HLN27AFX2_n01_AH10820_4h_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLN27AFX2_n01_AH10820_4h_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8622728
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT6411677.435779024921116TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT163350.18944120700548597TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTTT161350.18712175543517087TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTAT129160.1497901824109493TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT127710.14810858002247085TruSeq Adapter, Index 19 (97% over 40bp)
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG91260.10583657515347812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)