FastQCFastQC Report
Fri 15 Jan 2021
HLMNMAFX2_n02_vk12-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNMAFX2_n02_vk12-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6195704
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230280.3716768909554104No Hit
CGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG118460.19119699714511862Illumina Single End PCR Primer 1 (100% over 30bp)
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG110680.17863990920160164Illumina Single End PCR Primer 1 (100% over 32bp)
CGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG83440.13467396118342645Illumina Single End PCR Primer 1 (100% over 32bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC81870.13213994729251108Illumina Single End PCR Primer 1 (100% over 49bp)
GGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG80320.12963821383332708Illumina Single End PCR Primer 1 (100% over 41bp)
GTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT80250.12952523232226718Illumina Single End PCR Primer 1 (100% over 31bp)
AGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC78350.12645859130778359Illumina Single End PCR Primer 1 (100% over 38bp)
GGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT72680.11730708891192995Illumina Single End PCR Primer 1 (100% over 39bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT71970.11616113358546502Illumina Single End PCR Primer 1 (100% over 31bp)
AGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC71200.11491833696380589Illumina Single End PCR Primer 1 (100% over 40bp)
CGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGA66330.10705805183720848Illumina Single End PCR Primer 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)