FastQCFastQC Report
Fri 15 Jan 2021
HLMNMAFX2_n02_vk12-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNMAFX2_n02_vk12-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000561
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71850.35914925863295344No Hit
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG24510.12251563436456074Illumina Single End PCR Primer 1 (100% over 32bp)
GGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG23970.11981639150218365Illumina Single End PCR Primer 1 (100% over 41bp)
AGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC20010.10002194384475153Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)