FastQCFastQC Report
Fri 15 Jan 2021
HLMNMAFX2_n01_vk12-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNMAFX2_n01_vk12-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6195704
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG238560.38504098969221257Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC164660.26576479444466683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC155040.2502379067818605Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA153610.24792985591306493Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT141570.22849703601075844TruSeq Adapter, Index 9 (100% over 48bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137280.22157288340437178No Hit
CTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAG135440.21860308368508247Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC134260.2166985382129295Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA127640.20601371530983406TruSeq Adapter, Index 9 (100% over 40bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA125150.201994801559274TruSeq Adapter, Index 9 (100% over 38bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG120930.19518363046394727TruSeq Adapter, Index 9 (100% over 39bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC116060.1873233453373499TruSeq Adapter, Index 9 (100% over 37bp)
CACTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAGT101870.1644203790239172Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG100260.16182180426953902TruSeq Adapter, Index 9 (100% over 49bp)
ACACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTC92130.14869980877072242Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT87030.14046829867921387Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC84890.1370142924839534TruSeq Adapter, Index 9 (100% over 37bp)
CTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA83570.1348837839896806TruSeq Adapter, Index 9 (100% over 38bp)
CACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT79450.12823401505301094TruSeq Adapter, Index 9 (100% over 36bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT73710.11896953114609737TruSeq Adapter, Index 9 (100% over 36bp)
CACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTC71950.11612885315373361Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC71470.11535412279217988Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAG65580.10584753564728075Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACGACAAGATCGGAAGAGCACACGTCTGAACTCCAG65140.10513736614918984Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA63970.10324896089290256Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)