FastQCFastQC Report
Fri 15 Jan 2021
HLMNMAFX2_n01_vk12-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNMAFX2_n01_vk12-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789064
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55810.20010297361408702No Hit
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGG51200.18357413096293235TruSeq Adapter, Index 21 (100% over 40bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG47840.17152707861848993Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG46070.16518086354418543TruSeq Adapter, Index 21 (100% over 39bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC41030.14711028502752177Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC40340.14463633677821663TruSeq Adapter, Index 21 (100% over 38bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC38980.13976014892451374Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT38310.137357909320116TruSeq Adapter, Index 21 (100% over 37bp)
CTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAG34390.1233030149182665Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGGGGG33920.1216178617629427TruSeq Adapter, Index 21 (97% over 40bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC31170.11175792308817582Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC30970.11104083663910187TruSeq Adapter, Index 21 (100% over 38bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA30460.10921226619396328Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT29920.10727613278146361TruSeq Adapter, Index 21 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)