FastQCFastQC Report
Fri 15 Jan 2021
HLMNMAFX2_n01_vk12-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNMAFX2_n01_vk12-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5336768
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG71030.13309553647451042Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62030.11623139698034467No Hit
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAC59890.11222147936728746TruSeq Adapter, Index 19 (100% over 40bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC58240.10912972046002375Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA54410.10195309220861766TruSeq Adapter, Index 19 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)