FastQCFastQC Report
Fri 15 Jan 2021
HLMNMAFX2_n01_vk12-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNMAFX2_n01_vk12-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000561
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA42940.21463979353791263TruSeq Adapter, Index 18 (100% over 40bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG37240.1861477855461543TruSeq Adapter, Index 18 (100% over 38bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG36480.1823488511472532Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGC36440.18214890723152155TruSeq Adapter, Index 18 (100% over 39bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC32940.16465381460500328TruSeq Adapter, Index 18 (100% over 37bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC32590.16290430534235148Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29920.14955804896726468No Hit
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC27250.1362117925921779TruSeq Adapter, Index 18 (100% over 37bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC26310.1315131105724844Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA25240.12616461082666314Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG24250.1212159989123051TruSeq Adapter, Index 18 (100% over 38bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT23140.11566755525075217Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC22730.11361813011450288Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAG22570.11281835445157633Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTC22420.1120685647675827TruSeq Adapter, Index 1 (97% over 36bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTC22200.1109688732310587TruSeq Adapter, Index 1 (97% over 36bp)
CACTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAGT20080.10037184569728191Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)