FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_top2flow.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_top2flow.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6954
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC1782.5596778832326716TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAAATCTCGTATG751.0785159620362381TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCAAATCTCGTATG450.6471095772217429TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGATCGGAAGAGCAC440.632729364394593TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC420.6039689387402933TruSeq Adapter, Index 14 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260.37388553350589593No Hit
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACAC230.3307448950244464TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTGTGCTCTTCCGAT200.2876042565429969TruSeq Adapter, Index 8 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGGAAGAGCACACGT190.273224043715847TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCGGTCACAGATCGGAAGAGCACAC130.18694276675294796TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACAGATCGGAAGAGCACAC110.15818234109864826TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACAC110.15818234109864826TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTGAATCTCGTATGC70.10066148979004888TruSeq Adapter, Index 8 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAG405.293259E-945.00000430
TCACAGA206.5599376E-445.00000431
CAAATCT206.5599376E-445.00000439
AATCTCG250.00196657236.041
ACAGATC250.00196657236.033
AAATCTC250.00196657236.040
GTCAAAT250.00196657236.037
TCAAATC250.00196657236.038
AGGTCAA250.00196657236.035
CACAGAT250.00196657236.032
CCAGTCA701.2732926E-1135.35714327
TCCAGTC701.2732926E-1135.35714326
ACGTCTG801.8189894E-1233.75000416
CACGTCT801.8189894E-1233.75000415
GTCTGAA801.8189894E-1233.75000418
CTGAACT801.8189894E-1233.75000420
GAACTCC801.8189894E-1233.75000422
TCTGAAC801.8189894E-1233.75000419
CGTCTGA801.8189894E-1233.75000417
CAGTCAC752.910383E-1133.028
Produced by FastQC (version 0.11.2)