FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_top2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_top2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24817803
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC259876710.471382176738206TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG703720.28355451125145925No Hit
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT481510.19401797975429172TruSeq Adapter, Index 12 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC297180.11974468489414636TruSeq Adapter, Index 12 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2863550.043.06165345
TCGTATG2932850.042.0358844
CTCGTAT2913550.042.0104343
TCTCGTA2918500.041.8505242
AATCTCG2916900.041.85050640
ATCTCGT2920250.041.8039541
GTAATCT3172400.038.39435638
TAATCTC3176800.038.3829639
TGTAATC3184600.038.2153237
TTGTAAT3411950.035.9277636
CTTGTAA3571750.034.36276235
CCTTGTA3703300.033.1961234
CGGGGGG27550.031.7674985
TCGGGGG28000.031.4177134
ACCTTGT3954350.031.08549733
CACCTTG4025100.030.57555832
TCACCTT4114050.029.93829731
GTCACCT4176300.029.47779730
AGTCACC4242400.029.05874829
CAGTCAC4419600.027.94310428
Produced by FastQC (version 0.11.2)