FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_tls94c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_tls94c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13508261
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC919550.6807315908391169TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG121350.035.96863640
CGTATGC128150.034.49895545
CTCGTAT130050.033.78770443
ATCTCGT131850.033.24099741
TCGTATG134500.032.82013344
GCTTATC135500.032.26219637
TCTCGTA145050.030.35556642
TAGCTTA148700.029.59499535
AGCTTAT151800.028.9906236
TTATCTC154750.028.35084339
CTTATCT160950.027.3006738
CACTAGC166650.026.50258632
CTAGCTT166700.026.39937634
ACTAGCT171050.025.79425233
TCACTAG175100.025.1983931
AGTCACT180700.024.56807529
CAGTCAC182200.024.32903328
TCCAGTC188000.023.90170326
CCAGTCA187100.023.63183227
GTCACTA191350.023.15296230
Produced by FastQC (version 0.11.2)