FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_tls92c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_tls92c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22928928
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC1858290.8104565551429181TruSeq Adapter, Index 21 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG244200.035.323740
CTCGTAT250400.034.88020343
CGTATGC259800.033.9904145
ATCTCGT257600.033.5822841
TCGTATG270750.032.63249244
CGATCTC270600.031.85247239
TCGATCT271600.031.76004638
TCTCGTA280600.031.14220442
TTCGATC293400.029.4385137
CGTTTCG329450.026.6884834
GTTTCGA339200.025.91476435
AGTCACG378600.023.27374629
GTCACGT381950.023.08044230
TTTCGAT385450.022.8927236
ACGTTTC390350.022.59396733
CACGTTT394750.022.3425232
TCACGTT408150.021.64800531
CAGTCAC411200.021.47242428
TCCAGTC422700.021.05904626
CCAGTCA420200.021.02867527
Produced by FastQC (version 0.11.2)