FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_sgs1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_sgs1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18526821
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC1714970.9256687912081625TruSeq Adapter, Index 18 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG206350.037.310740
CGTATGC219250.035.54615845
CTCGTAT220850.035.08507543
GCATCTC220750.034.83607539
CGCATCT221350.034.74173738
CCGCATC225150.034.2152537
ATCTCGT226250.034.05874641
TCTCGTA226600.034.0359342
TCGTATG229850.033.89717544
TCCGCAT234800.032.96226536
GTCCGCA239700.032.37300535
CGTCCGC250750.030.95537234
ACGTCCG273000.028.46580333
CACGTCC293350.026.55288732
AGTCACG293300.026.53633129
TCACGTC296650.026.21277631
GTCACGT300550.025.91062730
CAGTCAC318500.024.51453228
CCAGTCA328800.023.78106527
TCCAGTC331600.023.74977126
Produced by FastQC (version 0.11.2)