FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_rpbdst.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_rpbdst.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20451448
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC221579110.834396664725157TruSeq Adapter, Index 11 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG443680.21694307415298908No Hit
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT349430.17085831770933774TruSeq Adapter, Index 11 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC212490.1038997336521111TruSeq Adapter, Index 11 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2400900.043.15323345
CATCTCG2404650.042.5961840
CTCGTAT2429200.042.45641743
TCTCGTA2426700.042.32956342
TCGTATG2445400.042.3203244
ATCTCGT2427300.042.25895341
ACATCTC2537400.040.37830739
TACATCT2564250.039.96857538
CTACATC2570950.039.84671437
GCTACAT2645050.038.79667736
GGCTACA2763850.037.26500335
CGGCTAC2868400.035.9618234
ACGGCTA3011950.034.3451533
CACGGCT3049150.033.92433532
TCACGGC3090600.033.47575831
GTCACGG3156450.032.8087630
AGTCACG3189150.032.51674729
CAGTCAC3313900.031.39120128
CCAGTCA3405250.030.57890527
CGGGGGG14250.030.4723955