FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_rpb9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_rpb9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19556493
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC1741760.8906300326955349TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC222650.037.08548745
CATCTCG218950.037.0133440
CTCGTAT232800.035.15930643
TCGTATG235100.035.02588344
ATCTCGT237850.034.22362541
TCTCGTA244950.033.314342
GTCCATC245100.033.1103237
CGTCCAT257000.031.7522936
CCGTCCA270850.030.17023735
CCATCTC280850.028.87961839
CCCGTCC287150.028.48897434
TCCATCT303400.026.87405838
ACCCGTC314950.026.13160133
TCACCCG322500.025.54233431
CACCCGT322450.025.51010132
GTCACCC340500.024.25834730
AGTCACC355800.023.27264629
CAGTCAC364250.022.85036528
TCCAGTC378550.022.35587926
CCAGTCA375000.022.28561227
Produced by FastQC (version 0.11.2)