FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_mrc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_mrc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16554872
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC7514414.539092781871101TruSeq Adapter, Index 13 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405600.24500340443586638No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC304750.18408478180924626TruSeq Adapter, Index 13 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC866350.040.75645445
CTCGTAT868650.040.10474843
TCGTATG887800.039.78453444
ATCTCGT874850.039.72023441
TCTCGTA879450.039.53549642
AATCTCG880500.039.43189640
AAATCTC1025300.033.8719339
CAAATCT1031700.033.64654538
TCAAATC1057550.032.7879537
GTCAAAT1090750.032.08698336
AGTCAAA1148050.030.58544335
CAGTCAA1193750.029.49559234
ACAGTCA1272850.027.6574833
CACAGTC1281250.027.48702432
TCACAGT1303350.027.08439831
CGGGGGG13000.026.8256175
GTCACAG1374650.026.0025130
TCGGGGG13700.025.9476414
AGTCACA1384550.025.57211929
GTATGCC77650.025.29466445
Produced by FastQC (version 0.11.2)