FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_mph1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_mph1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36819386
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC4783441.2991634352620656TruSeq Adapter, Index 14 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC465900.12653660221275825TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG387070.10512668516525507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG572850.038.50559640
CGTATGC596300.037.8437945
CTCGTAT607050.036.77365543
TCGTATG621550.036.23411644
ATCTCGT613900.036.01144841
TCTCGTA635250.034.94635842
CCATCTC734800.030.07411439
GTTCCAT750100.029.66448436
TCCATCT773050.028.63554438
AGTTCCA785950.028.5089835
CAGTTCC813000.027.61862434
TTCCATC811500.027.32581137
GTCACAG946750.026.58958830
ACAGTTC853050.026.39601733
AGTCACA951300.026.01848829
TCACAGT872200.025.91314131
CACAGTT874300.025.89634532
CAGTCAC988650.025.09026728
CCAGTCA1110800.022.33128527
TCCAGTC1123050.022.23022826
Produced by FastQC (version 0.11.2)