FastQCFastQC Report
Wed 5 Oct 2016
HLMNCBGXY_n01_cdc13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMNCBGXY_n01_cdc13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19387618
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC1856500.9575699294260904TruSeq Adapter, Index 22 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC238050.037.98439445
CTCGTAT245300.036.48578643
TCGTATG250450.036.13070344
GATCTCG245600.035.8000640
ATCTCGT247500.035.55250541
TCTCGTA253450.035.12612542
TACGATC270400.032.4751937
ACGATCT273750.032.13530738
CGATCTC294400.029.85060739
GTACGAT303150.029.21908436
CGTACGA324400.027.49926235
CCGTACG358500.025.10956634
ACCGTAC415950.021.71761333
CACCGTA425050.021.27961732
TCACCGT439700.020.6119831
GTCACCG446200.020.2918630
AGTCACC449100.020.21651329
CAGTCAC471650.019.53151728
TCCAGTC494500.019.06142826
CCAGTCA486550.019.00295627
Produced by FastQC (version 0.11.2)