FastQCFastQC Report
Sat 20 Nov 2021
HLMHLDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLMHLDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922950580
Sequences flagged as poor quality0
Sequence length8
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTAGA14989205816.240529151625864No Hit
CAGTACTG14744489515.975383535703505No Hit
AGTAGTCT14263867715.454638643815578No Hit
TTCCCGAC13798375114.95028596222346No Hit
GGGGGGGG11309519012.25365609499915No Hit
CAACGCCT653599287.081628140913026No Hit
TGTGCGGG642895976.965659743125141No Hit
ACCATAAC545535625.910778234734952No Hit
GTGTATTA207282892.2458720379156163No Hit
TTCCGACA13854140.1501070620704307No Hit
GGGTATTA13692660.14835745593225588No Hit
TCCCGACA9646340.10451632198985128No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)